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College of Agriculture
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VMB
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Faculty & Research
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Dr. Radke
Department of Veterinary Molecular Biology
Jay Radke, Assistant Professor
Veterinary Molecular Biology Laboratory
Center for Immunotherapies to Zoonotic Disease
P.O. Box 173610
Montana State University
Bozeman, MT 59717
(406) 994-3799
jradke@montana.edu
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Research Interests
We seek to identify and study host-pathogen interactions that are critical to the progression of disease caused by two obligate intracellular pathogens: (1) Toxoplasma gondii and (2) Mycobacterium avium ssp. paratuberculosis (MAP). T. gondii is a member of a diverse family of protozoans responsible for diseases that range from malaria (Plasmodium ssp.) in human populations throughout Africa and southeast Asia, to opportunistic infections associated with AIDS and premature birth or congenital birth defects in human populations (Toxoplasma), to significant intestinal disease in commercial cattle herds (Eimeria ssp., Cryptosporidium ssp.). MAP is a bacterial pathogen that has been associated with the chronic imflammatory bowel syndrome known as Crohn's Disease, but also infects wild and domestic ruminants causing similar clinical pathologies (Johne’s Disease). In all, these pathogens have distinct affinities for tissues and cell types in the host, but the molecular interactions required to establish persistent infection are largely unknown. We believe that obligate intracellular pathogens have evolved finite strategies to establish infection and cause disease. Thus, detailed studies of diverse pathogens will uncover global similarities in host-pathogen communication and provide the basis for new therapeutics with the potential to be efficacious across diverse genre. Our research seeks to identify both host- and pathogen-specific biochemical pathways altered by interaction of the microbe and its host cell and to characterize the molecular interactions able to influence the pathogenesis of disease. In this context, we are investigating global host cell gene expression in both the pathogen and the infected cell; and using siRNA technology and tetracyline-regulated gene expression in model host cells, as well as genetic strategies in the microbe itself, to test role of selected mRNA(s) and proteins during infection.
Education
- Ph.D. Biochemistry, Montana State University, 1996
- M.Ed. Computer Science, Eastern Montana College, 1990
- B.S. Biology, Eastern Montana College, 1986
Professional Experience
Montana State University-Bozeman. Host-parasite gene expression in Toxoplasma-infected cells.
Extramural Grant Activity
This work is funded by the March of Dimes, the USDA-NRI, and the NIH.
Selected Publications
- Radke J. R, and Boyle, J. (2009). A history of studies to examine the interactions of Toxoplasma with its host cell: Emphasis on in vitro models. Int. J. Parasitol. (Eds. Jim Ajioka and Naomi Morrissette). July 39(8) 903-914.
- White, M. W., Radke J. R, Conde de Philip, M., Lehman, M. (2007). Cell Cycle Control and Parasite Division. In: “The Biology of Toxoplasma gondii” (Eds. Jim Ajioka and Dominique Soldati). Horizon Scientific Press, Hethersett, Norwich, UK.
- Radke, J. R., Eibs, C. A. and Fox, P. D. (2007). Host Cell-directed Interactions with Toxoplasma Influence Pathogenesis: The host molecular environment can influence parasite growth and cyst development. Microbe May;2(5) p 34.
- White, M. W., Sullivan, W. J. Jr., and Radke J. R. (2007). Gene Regulation. In: “Toxoplasma gondii: The Model Apicomplexan: Perspective and Methods” (Eds. Kami Kim and Louis Weiss). Academic Press, 84 Theobald’s Road, London, UK.
- Radke, J., Donald, R. G., Eibs, C. A., Jerome, M., Behnke, M., Liberator, P. A. and White, M. W. (2006). Changes in the expression of human cell division autoantigen-1 influences Toxoplasma gondii growth and development. PLoS Pathogens Oct;2(10):e105.
- Graff, J. C., Behnke, M., Radke, J. White, M. W., Lee, D. and Jutila, M. A. (2006). A comprehensive SAGE database for the analysis of gamma-delta T cells Int. Immunol. Apr;18(4):613-26.
- Radke, J., Behnke, M., Mackey, A., Radke, J. B., Roos D. S., and White, M. W. (2005). Construction of a developmental transcriptome for Toxoplasma gondii. BMC Biol. Dec 2, 3:26.
- Rider, S. D., Cai, X., Sullivan, W. J., Smith, A. T., Radke, J., White, M. W. and Zhu, G. (2005). The protozoan parasite Cryptosporidium parvum possesses two functionally and evolutionarily divergent replication protein A large subunits. J. Biol. Chem. 280(36):31460-31469.
- Radke, J., Gubbels, M.-J., M., Jerome, M., Striepen, B. and White, M. W. (2004). Isolation of sporozoite surface antigen by genetic complementation in Toxoplasma gondii. Mol Microbiol. Apr;52(1):93-105.
- Radke, J., Guerini, M., Jerome, M. and White, M. (2003). A change in the premitotic period of the cell cycle is associated with bradyzoite differentiation in Toxoplasma gondii. Mol. Biochem. Parasitol. Oct;131(2):119-27.
- Li, L., Brunk, B. P., Kissinger, J., Pape, D., Martin, J., Wylie, T., Dante, M., Tang, K., Cole, R., Fogarty, S. J., Howe, D., Liberator, P., Diaz, C., White, M., Jerome, M., Johnson, E., Radke, J. R., Waterston, R., Clifton, S., Roos, D. S. and Sibley, L. D. (2003). Gene discovery in the Apicomplexa as revealed by EST sequencing and assembly of a comparative gene database. Gen. Res. 13:443-454.
- Schmidt, E., Bondareva, A., Radke, J. and Capecchi, M. (2003). Fundamental cellular processes do not require vertebrate-specific sequences within the TATA-binding protein. J. Biol. Chem. Feb 21;278(8):6168-74.
- Meissner, N., Radke, J., White, M., Behnke, M., Hedges, J., Abrahamsen, M. and Jutila, M. (2003). Serial analysis of gene expression in circulating gamma delta T cell subsets defines distinct immunoregulatory phenotypes and unexpected gene expression profiles. J. Immunology. Jan 1;170(1):356-64.
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