Mark Jutila, Professor

Veterinary Molecular Biology P.O. Box 173610 Montana State University Bozeman, MT 59717 (406) 994-4540 uvsmj@montana.edu

Research Interests

Inflammatory disease and developmental immunology. My laboratory studies the molecular events that control leukocyte entry into sites of acute and chronic inflammation. Analyses of gamma/delta T cells are also pursued in the context of host immune responses and developmental immunology.

Jutila Laboratory Home Page

Education

• B.S. Montana State University, Bozeman, 1982, Microbiology
• M.S. Washington State University, Pullman, 1984, Microbiology
• Ph.D. Washington State University, Pullman, 1986, Immunology/Vet Science
• Postdoctoral Stanford University, Stanford, California, 1989, Immunology

Professional Experience

• 2001 - Present. Affiliate Professor, Department of Immunology, University of Washington. Seattle, WA
• 2000-present, Professor, Veterinary Molecular Biology, Montana State University-Bozeman
• 1995-2000, Head/Associate Professor, Veterinary Molecular Biology, Montana State University-Bozeman
• 1994-1995, Acting Head, Veterinary Molecular Biology, Montana State University-Bozeman
• 1989-1995 Assistant Professor, Immunology, Montana State University-Bozeman
• 1986-1989, Postdoctoral Fellow, Department of Pathology, Stanford University School of Medicine
• 1986, Research Assistant, Veterinary School, Washington State University
• 1982-1986, Teaching Assistant, Washington State University

Honors and Awards

• Senior Postdoctoral Fellowship, American Cancer Society, California Division, 1988-1989.

• Postdoctoral Fellowship, NIH Training Grant, Dept. of Path., Stanford School of Medicine, 1986-1988.

• Charles and Nora L. Wiley Award for Meritorious Research, Montana State University, 1993.

• Alberta Heritage Foundation Visiting Scientist, Calgary, Alberta,  1994.

• Invited lecturer, Washington State University, 1998

• MSU Alumni Association/Chamber of Commerce Award for Excellence, 1999

• Cox Award for Creative Scholarship and Teaching,  Montana State University, 2001.

• Meritorious Technology/Science Award, 2005 MSU.

• Distinguished Veterinary Immunologist of the year, AAVI, 2006.

• Annual WWAMI Science in Medicine Lecture, University of Washington School of Medicine, 2009

Service & Memberships:

• U.S.D.A. Animal Growth Review Panel, 1991 and 1992.

• Ad-Hoc reviewer for NIH, 1992.

• Member, Editorial Board of Transplantation, 1992-2008. 

• Member of  AAI.   Associate Editor, J. Immunol., 1994-1998

• U.S.D.A. NRI Grant Review Panel Member, 1996,1997,1998, 2000, 2002, 2004.

• NIH PPG Panel member, 2000-2001

• American Heart Assoc. grant review panel member, 2000-2001

• Section Editor J. Immunology, 2001-2006

Extramural Grant Activity

• NIH Rocky Mountain RCE(PI:J. Belisle)                                                                                            

  Region VIII RCE

  Dr. Jutila is PI on a Subproject entitled: Enhancement of innate immunity to Coxiella pneumonia.  This project is focused on identification of innate adjuvants that increase macrophage killing of C. burnetii.

• NIH NIAID N01-AI-400009 (Jutila, PI)                                       

  NIH NIH-NIAID                                                                          

  Title: Innate Immune Receptors and Adjuvant Discovery. The goal of this project is to begin to understand the repertoire of innate receptors expressed by human T cells that can be targeted in the development of new therapeutic adjuvants.  This program is focused on drug discovery.

• RR020185                                                                                

  NIH (PI: M Quinn)                                                                     

  Title:  Center for zoonotic infectious diseases.  Program is focused on new faculty hires and their professional development.  Dr. Jutila is a co-PI, Director of the Cell Biology Core, and faculty mentor.

   

• NIH/NCCAM P01 AT004986                                                    

  PO1 Director: M. Jutila,  Project 1 PI: M. Jutila                        

  Title: CAMS as countermeasures against infectious and inflammatory disease.  Three subprojects.  Dr. Jutila is the PI for the overall program and directs one of the subprojects on the effect of complementary

  and alternative medicines that affect γδ T cells.

Selected peer-reviewed publications from 100 total (in chronological order). 

1. Bargatze, R., Jutila, M.A., and Butcher, E.C. 1995. Distinct roles for L-selectin,  a4 integrin, and LFA-1 in lymphocyte interactions with HEV in situ: the multi-step model confirmed and refined. Immunity, 3:99.

2. Burritt, J.B., Quinn, M.T., Jutila, M.A., Bond, C.W., Jesaitis, A.J.  1995.  Topological mapping of neutrophil cytochrome B epitopes with phage-display libraries.  J. Biol. Chem., 270:16974.

3. Jutila, M.A. and Kurk, S.  1996.  Analysis of bovine γ/δ T cell interactions with E-, P-, and L-selectin:  Char-acterization of lymphocyte on lymphocyte rolling and the effects of O-glycoprotease.  J. Immunol. 156: 289.

4. Hickey, M.J., Reinhardt, P.H., Ostrovsky, L. Jones, W.M., Jutila, M.A., Payne, D., Elliot, J., and Kubes, P. 1997. Tumor necrosis factor-alpha induces leukocyte recruitment by different mechanisms in vivo and in vitro.  J. Immunol.,  158:3391.

5. McEvoy, L.M., Jutila, M.A., Tsao, P.S., Cooke, J.P., Berg, E.L., and Butcher, E.C. 1997.  Anti-CD43 inhibits monocyte-endothelial cell adhesion in inflammation and atherogenesis.  Blood, 90:3587.
6. Jones, W.  Watts, G., Robinson, M., Vestweber, D., and Jutila, M.A. 1997.  Comparison of E-selectin binding glycoprotein ligands on human lymphocytes and neutrophils, and bovine gamma/delta T cells.  J. Immunol.,  159: 3574.

7. Jutila, M.A., Wilson, E., and Kurk, S. 1997. Identification and characterization of an adhesion molecule that mediates leukocyte rolling on 24-hour cytokine stimulated bovine endothelial cells under flow conditions. J. Exp. Med.,  186:1701.

8. Kahn, J., Walcheck, B., Migaki, G.I., Jutila, M. and Kishimoto, T.K. 1998. Calmodulin regulates L-selectin adhesion molecule expression and function through a protease-dependent mechanism: Association of calmodulin with the cytoplasmic tail of L-selectin.  Cell, 92: 809.

9. Jones, W.  Watts, G., Robinson, M., Vestweber, D., and Jutila, M.A. 1997.  Comparison of E-selectin binding glycoprotein ligands on human lymphocytes and neutrophils, and bovine gamma/delta T cells.J. Immunol.,159: 3574.
10. Jutila, M.A., Wilson, E., and Kurk, S. 1997. Identification and characterization of an adhesion molecule that mediates leukocyte rolling on 24-hour cytokine stimulated bovine endothelial cells under flow conditions. J. Exp. Med.,  186:1701.
11. Leid, J.G., Speer, C.A., and Jutila, M.A.  1998. Comparative ultrastructure and expression of L-selectin on bovine ab and γδ T cells.  J. Leuk. Biol.   64:104.
12. Wilson, E., Aydintug, K., and Jutila, M.A. 1999. A circulating γδ T cell subset, which is found in large numbers in spleen, accumulates inefficiently at sites of inflammation: correlation with lack of E-selectin ligands and L-selectin.  J. Immunol., 162:4914.
13. Csencsits, K.L., Jutila M.A., and Pascual D.W.  1999.  Nasal-associated lymphoid tissue (NALT): Phenotypic and functional evidence for the primary role of peripheral node addressin in naïve lymphocyte adhesion to HEV in a mucosal site.  J. Immunol., 163: 1382.
14. Kantele, J., Kurk, S., and Jutila, M.A. 2000. Effect of continuous exposure to SDF-1a on T cell rolling and tight adhesion to monolayers of activated endothelial cells.  J. Immunol. 164: 5035.
15. Leid, J.G., Steeber, D.A., Tedder, T.F., and Jutila, M.A. 2001.  Antibody binding to a conformation-dependent epitope  induces L-selectin associate with the detergent resistant  cytoskeleton.J. Immunol., 166:4899.

16. Quinn, M.T., Swain S.D., Parkos C.A., Jutila K.L., Siemsen D.W., Kurk, S.L., Jesaitis A.J., and Jutila M.A. 2001.  A carbohydrate neoepitope that is up-regulated on human mononuclear leukocytes by neuraminidase treatment or cellular activation.  Immunology, 104: 185.

17. Leid, J.G., Speer, C.A., and Jutila, M.A. 2002. Ultrastructural Examination of Cytoskeletal Linkage of L-selectin and Comparison of L-selectin Cytoskeletal Association to that of Other Human and Bovine Lymphocyte Surface Antigens. Cellular Immunology,  215: 219..

18. Csencsits, K.L., Jutila, M.A., and Pascual, D.W. 2002.  Mucosal addressin (MAdCAM-1) expression and naïve lymphocyte binding interactions vary among the cranial, oral, and nasal-associated lymphoid tissues (CONALT).Eur. J. Immunol., 32:3029.

19. Jutila, M.A., Kurk S., Jackiw L., Knibbs R.N., and Stoolman, L.M. 2002. L-selectin can serve as an E-selectin ligand on human T-cell blasts. J. Immunol.  169: 1768.

20. Wilson, E., Hedges J. F., Butcher E.C., Briskin M., and Jutila, M.A. 2002.  Bovine γδ T cell subsets express distinct patterns of chemokine responsiveness and adhesion molecules: A mechanism for tissue specific γδ T cell subset accumulation.  J. Immunol., 169: 4970.

21. Meissner N., Radke J., Hedges J. F. White M., Behnke M., Bertolino S., Abrahamsen M. Hedges, and Jutila, M.A. 2003. Serial analysis of gene expression (SAGE) in circulating  T subsets defines distinct immunoregulatory phenotypes and unexpected gene expression profiles. J. Immunol. 170: 356.

22. Hedges, J. F., Cockrell, D., Jackiw, L.,  Meissner, N., and Jutila, M.A. 2003. Differential mRNA expression in circulating γδ T lymphocyte subsets defines unique tissue-specific functions.  J. Leuk. Biol., 73: 306

23. Hedges, J.F., Graf, J, and Jutila, M.A. 2003.  Transcriptional profiling of gamma/delta T cells.  J. Immunol. 171: 4959.

24. Leid, J. and Jutila, M.A. 2004. Impact of Polyunsaturated Fatty Acids on Cytoskeletal linkage of L-selectin. Cellular Immunol, 228: 91.

25. Hedges J. F., Lubick, K., and Jutila, M.A. 2005. γδ T cells respond directly to pathogen-associated microbial products.  J. Immunol. 174: 6045.

26. Graff, J.C., Behnke, M., Radke, J., White, M., and  Jutila, M.A. 2006.  A comprehensive SAGE database for the analysis of γδ T cells. Internal. Immunol., 18: 613.

27. Lubick, K. and Jutila, M.A. 2005. LTA recognition by γδ T cells involves CD36. 2006. J. Leuk. Biol., 79: 1268.

28. Lahmers K.K., Hedges J. F., Jutila M.A., Deng M., Abrahamsen M.S. and Brown W. C. 2006. Comparative gene expression by WC1+ gammadelta and CD4+ alphabeta T lymphocytes, which respond to Anaplasma marginale, demonstrates higher expression of chemokines and other myeloid cell-associated genes by WC1+ gammadelta T cells.J. Leuk. Biol., 80:939.

29. Kress, E., Hedges, J.F., and Jutila, M.A. 2006. Distinct gene expression in human Vδ1 and Vδγδ T cells following non-TCR agonist stimulation.  Mol. Immunol., 43: 2002.

30. Shaneyfelt, M., Burke, A.D., Jutila, M.A., and Hardy, M.E. 2006. Natural products that reduce rotavirus infectivity identified by a cell-based semi-high-throughput screening assay.Virology J., 3:68.

31. Buckner, D., Wilson, S., Kurk, Hardy, M., Miessner, N. and Jutila, M.A. 2006. Use of early passage fetal intestinal epithelial cells in semi-high throughput screening assays: an approach to identify new innate immune system adjuvants. J. Biomol. Screen, 11:664.

32. Uchida, M.,  Flenniken, M., Allen, M., Willits, D.A., Crowley, B.E., Brumfield, S., Willis, A.F., Jackiw-Turk, L., Jutila, M., Young, M. and  Douglas, T. 2006. Targeting of cancer cells with ferrimagnetic ferritin cage nanoparticles. J. Amer. Chem. Soc.,128:16626.

33. Kaiser, C.R., Flenniken, M., Harmsen, A.G., Harmsen, A. L.,  Jutila, M.A., Douglas, T.,and Young, M.J. 2007. Biodistribution studies of protein cage nanoparticles demonstrate broad tissue distribution and rapid clearance in vivo. Intern. J. Nanomedicine, 2: 1.

34. Hedges, J.F., Buckner, D., Kerns, H., Rask, K., Jackwi, L. Theresa Trunkle, David Pascual and Jutila, M.A. 2007. Mucosal lymphatic-derived γδ T cells respond early to experimental Salmonella enterocolitis by increasing expression of IL-2Ra.  Cell. Immunol., 246: 8.

35. Lubick, K., Radke, M., and Jutila, M.A. 2007. Securinine, a GABAA receptor antagonist, enhances macrophage clearance of phase II C. burnetii: comparison to TLR agonists.  J. Leuk. Biol.  82:1062.

    Graff, J. and Jutila, M.A. 2007. The differential regulation of CD11b on T cells and monocytes in response to unripe apple polyphenols.  J. Leuk. Biol., 82: 603.

36. Holderness, J., Jackwi, L., Radke, M., Kerns, H., Hedges, J., Kimmel, E., Petrie, C., McCurley, P., Glee, P., Palecanda, A., and Jutila, M.A. 2007. Select plant polyphenols (condensed tannins) enhance IL-2R? upregulation and cell division in γδ T cells.  J. Immunol., 179: 6468.

37. Jutila MA, Walcheck B, Bargatze R, and Palecanda A. 2007. Measurement of neutrophil adhesion under conditions mimicking blood flow. Methods Mol Biol.  412:239-56.

38. Ren, Y., Strobel, G., Graff, J., Jutila, M., Park, S.G., Gosh, S., Teplow, D., Condron, M., Pang, E., and Hess, W.M. 2008.  Colutellin A, an immunosuppressive peptide fromColletotrichum dematium, Microbiology, 154: 1973-1979.

39. Jutila, M.A., Holderness, J., Graff, J. and Hedges, J.F. 2008. Antigen independent priming:  A transitional response of bovine ?? T cells to infection.  AHRP, 9: 47-57.

40. Nemali S., Siemsen, D.W., Nelson, L.K., Bunger, P.L., Faulkner, C.L., Rainard, P., Gauss, K.A., Jutila, M.A., Quinn MT. 2008. Molecular analysis of the bovine anaphylatoxin C5a receptor. J. Leukoc Biol. 84:537-549.

41. Schepetkin, I.A., Xie, G., Kirpotina, L.N., Jutila, M.A., and Quinn, M.T. 2008. Macrophage immunomodulatory activity of polysaccharides isolated from Opuntia polyacantha. Int. Immunopharmacol. 8: 1455-1466 .

42. Holderness, J., Hedges, J., Daughenbaugh, K., Kimmel, E., Graff, J., Freedman, B., and Jutila, M.A.  2008. Response of gamma/delta T cells to plant-derived tannins.  Critical Rev. Immunol.,  28: 377-402.

43. Schepetkin, I.A., Xie, G., Jutila, M.A., and Quinn, M.T. 2009. Complement-fixing Activity of Humic Substances from Shilajit and Other Natural Sources. Phytother. Res., 23: 373-384.

44. Kerns, M.M., Jutila, M.A., and Hedges, J.F.  2009.  The distinct responses of γδ T cells to the Nod2 agonist, muramyl dipeptide.  Cellular Immunol in press.

45. Daughenbaugh, K., Hedges, J., Graff, J., Graf, J., Holderness, J., and Jutila, M.A. 2009. Increased immune cytokine mRNA stability: a novel effect of plant-derived tannins on gamma/delta T cells. Submitted.

46. Graff, J., Kimmel, E.M., Schepetkin, I.A., Freedman, B., Lubick, K., Holderness, J., Quinn, M.T., Jutila, M.A. and Hedges, J.T. 2009. Priming of gamma/delta T cells by polysaccharides from YamoaTM, a unique immunomodulatory dietary supplement. Submitted.

47. Siemsen, D.W., Jutila, M.A., and Quinn, M.T. 2009.  Coxiella burnetii Phase II inhibits human neutrophil NADPH oxidase assembly.  Microbes and Infect., In press.

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